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Geneva8* 2  AUTOSAVE.WKZ!1 <d 8# 0 \\H\ H  v \< @       !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcd@ A@ B@ ex@  em@  of@  ema@ emb@  exa@ exb@  epsa@ epsb@ raman@ snr@ xraman@  Aex@ Aem@RamInt@xRamInt@h1ax@p1ax@w1ax@h2ax@p2ax@w2ax@h3ax@p3ax@w3ax@h1am@p1am@w1am@h2am@ p2am@ !w2am@!"h3am@"#p3am@#$w3am@$%h1bx@%&p1bx@&'w1bx@'(h2bx@()p2bx@)*w2bx@*+h3bx@+,p3bx@,-w3bx@-.h1bm@./p1bm@/0w1bm@01h2bm@12p2bm@23w2bm@34h3bm@45p3bm@56w3bm@ 6 Tex@ 7Tem@G+y@%-9@%@42333333?+u@%-9@%@420.333333?+r@%-9@%@420.%+y@%-9@%@42333333?+ |@%-9@%@420.333333?+@@%-9@%@420.%0@ c@#G+y@%-9@%@42333333?+u@%-9@%@420.333333?+r@%-9@%@420.%+y@%-9@%@42333333?+ |@%-9@%@420.333333?+@@%-9@%@420.%0%+@z@%-9@%@42333333?+ w@%-9@%@420.333333?+t@%-9@%@420.%+@z@%-9@%@42333333?+`}@%-9@%@420.333333?+@@%-9@%@420.%0%.@ G@Gĝ+?%0.@@@ 0@ 0   /% 1%@420   /% 1%@420.   /% 1%@420.%0 0 $  %/% &1%@420 '  (/% )1%@420. *  +/% ,1%@420.%0.@@@ 0@ @+ G*@% 1%0.%0@ ĝ+?%0.@ I$@2%4@  0   /% 1%@420   /% 1%@420.   /% 1%@420.%0 0 $  %/% &1%@420 '  (/% )1%@420. *  +/% ,1%@420.%0.@! ? ,q@H6?/%1@` -  ./% /1%@420 0  1/% 21%@420. 3  4/% 51%@420.%@` $  %/% &1%@420 '  (/% )1%@420. *  +/% ,1%@420.%@4G 60   /% 1%@420  /% 1%@420. ! 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"/ .% #1%@420.%0%0  $ %/% &1%@420 ' (/% )1%@420. * +/% ,1%@420.% - ./ .% /1%@420 0 1/ .% 21%@420. 3 4/ .% 51%@420.%0%0.Y@ %-$@%@420.  .?,q@H6?/%1%/%-$@%@420.%0@G$@ 2 0  / .% 1%@420  / .% 1%@420.  / .% 1%@420.%0 0 $ / .% &1%@420 ' / .% )1%@420. * / .% ,1%@420.%0.%4@  +y@%-9@%@42333333?+u@%-9@%@420.333333?+r@%-9@%@420.%+y@ .%-9@%@42333333?+ |@ .%-9@%@420.333333?+@@ .%-9@%@420.%0%0 +@z@%-9@%@42333333?+ w@%-9@%@420.333333?+t@%-9@%@420.%+@z@ .%-9@%@42333333?+`}@ .%-9@%@420.333333?+@@ .%-9@%@420.%0%0.Y@ %-$@%@420. ?,q@H6?/%1%/%-$@%@420. @ \ Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @  Geneva @)  Times New Roman @Excitation wavelength53z)r@&@P,6sp@? @fEmission wavelengthy@35N{p@ @SSynchronous Spectrum @F  @F? @  Excitation  Emission   Wavelength? @  Wavelength   Wavelength  Offsetraman,x- RamInt,x- @ 53z)r@nm y@nm 98"@nm%-# 7't@%C% <@ @      Absorbance%9hv@%@@ Concentration, ppm @  $Concentrations of components A and B  % ۢ&Tqc?o#sD? @  [A] [B]  IntensityMbP?MbP?;? @  ?ppm ppm% %7jp@% %7jp@Mb`?Mb`?%5? @ %"G6p@ema, embexa, exb epsa, epsbAex, AemTex,Tem{Gzt?{Gzt?u7N? @ %a-?%VX?@h㈵>%H+y׆e?% ):!?{Gz?{Gz?m @ @%ɓ?% cz?@h㈵>% ig$?% ;n?{Gz?{Gz?aWj&@ @ Sample solution insertedppb Appb BSNR??GTh?+@ @%@@%@??;@ @c??ݮjJ@ @uband parameters??4p`@ @th1ax ???Vp@ @tp1ax `x@@@@#9@ @tw1ax 4@@@Ih@ @th2ax 333333?$@$@-e@ @p2ax @u@4@4@ḩ@ @Version 2.1: June 9, 2000w2ax 9@I@I@1sm]K@ @h3ax 333333? 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W^@%I$I${@%zg F@%I$I${@% x.G@ @H%t?%da4>>% QǢ?{@%0?%{@%_,?%{@%|R1a@%{@%hb@%{@%@E@%{@% y[E@ @I%WP?%u8>% (i?m۶m{@%[FHn ?%m۶m{@%= ?%m۶m{@%L;4d@%m۶m{@%Aͽd@%m۶m{@%'ؠD@%m۶m{@% kƸ D@ @J%g?%M!F֪>% e7#?n۶m6|@%b0l>%n۶m6|@%8*p{?%n۶m6|@%5'e@%n۶m6|@%iCve@%n۶m6|@% g D@%n۶m6|@% [.E@ @K%_?%S\ >% {sb?%I$I|@%Y€M>%%I$I|@%ڷ?%%I$I|@%5Ua`od@%%I$I|@%8qB|d@%%I$I|@%:~D@%%I$I|@% \{iE@ @L%It?%Aq?R>% ?ܶm۶|@%z~0,>%ܶm۶|@%B?%ܶm۶|@%V9jb@%ܶm۶|@%>lb@%ܶm۶|@%ͺ?~D@%ܶm۶|@% LpE@ @M%/?%5-!>% ?$I$I}@%^2&,?%$I$I}@%~HE?%$I$I}@%o_@%$I$I}@%l `@%$I$I}@%$~D@%$I$I}@% HMDE@ @N%?% ==% ?I$I}@%m sjs?%I$I}@%_IjͰ?%I$I}@%j\E^[@%I$I}@%?Z[@%I$I}@%I~D@%I$I}@% s D@ @O%(?%d[=% ?~@%N0W[?%~@%qQ?%~@%䫉}W@%~@%'"X@%~@%\~D@%~@% _/D@ @P%3?%.x s=% f?m۶m[~@%4n{?%m۶m[~@%ەc?%m۶m[~@%AZmSU@%m۶m[~@%V@%m۶m[~@%?X~D@%m۶m[~@% MAiE@ @Q%?%WG 3=% ?n۶m۶~@% x ?%n۶m۶~@%_DJ?%n۶m۶~@%Bv.U@%n۶m۶~@%;. U@%n۶m۶~@%٣X~D@%n۶m۶~@% (ۈE@ @R%?%cJ<% ?%I$I@%3}6W?%%I$I@%FH @%%I$I@%)͕T@%%I$I@% %T@%%I$I@%X~D@%%I$I@% J`1E@ @S%?%P`<% ?ܶm۶m@% !?%ܶm۶m@%28?%ܶm۶m@%S_1S@%ܶm۶m@%hT@%ܶm۶m@%d`X~D@%ܶm۶m@% U@uE@ @T%?%?,\ Y<% ?$I$@%T?%$I$@%,ms?%$I$@%/R@%$I$@%fgR@%$I$@%H^X~D@%$I$@% nȑD@ @U%?%U1<% ?%I$I@%-?%%I$I@%QY+T?%%I$I@%P@%%I$I@%$P@%%I$I@%6^X~D@%%I$I@% *E@ @V%?% w%;% ?@@%gɢ?%@@%?%@@%VT4L@%@@%۵PsoM@%@@%7^X~D@%@@% wvD@ @W%?%2+f^;% ?۶m۶m@%C/"L?%۶m۶m@%ЫF ?%۶m۶m@%WwG@%۶m۶m@%;lG@%۶m۶m@%7^X~D@%۶m۶m@% UD@ @X%?%J;% ?m۶m@%Щn>%m۶m@%hV ?%m۶m@%Db'kHB@%m۶m@%I*B@%m۶m@%7^X~D@%m۶m@% rn?hE@ @Y%?%G@:% ?$I$ɀ@%e>%$I$ɀ@%eSI9?%$I$ɀ@%Ȯ;@%$I$ɀ@%RյG<@%$I$ɀ@%7^X~D@%$I$ɀ@% :=9܉0E@ @Z%?%E@:% ?n۶m@% *`0>%n۶m@%` ?%n۶m@%*&2@%n۶m@%j43@%n۶m@%7^X~D@%n۶m@% p9D@ @[%?%„c9% ?I$I$@%A=%I$I$@%mޤ?%I$I$@% D(Eb)@%I$I$@%'ښ,*@%I$I$@%7^X~D@%I$I$@% CxD@ @\%?%E|%p9% ?%I$IR@%o2zv>%%I$IR@%D?%%I$IR@%6 @%%I$IR@% @%%I$IR@%7^X~D@%%I$IR@% M0E@ @]%?%Kv9% ?@% >%@% `?%@%|@%@%0_Ʌ@%@%7^X~D@%@% 5VE@ @^%?%%198% ?ܶm۶@% V]I?%ܶm۶@%> /6_( ?%%I$I@%4\t&?%%I$I@%7^X~D@%%I$I@% udyE@ @d%?%Cra6˼5% ?@%Y@%@%қj/Y@%@%Q¶?%@%ޯW?%@%7^X~D@%@% sTD@ @e0 Toff0 absorbance0 transmission0 wavelength0 ex0 0 ex+noise0 0 em0 0 em+noise0 0 synch0 0 synch+noise @Zf @Zg @Zh @Zi @Zj @Zk @Zl @Zm @Zn @Zo @Zp @Zq @Zr @Zs @Zt @Zu @Zv @Zw @Zx @Zy @Zz @Z{ @Z| @Z} @Z~ @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z @Z  ?9$@:    !.-@  *?on idle if z1 = 1 if W1 > 10 recalc range O8..S17 recalc range M10 put X1 into X2 put O12 into X1 put (X1+X2)/2 into X1 recalc range M12 put 0 into W1 end if put W1+1 into W1 end if end idle Uf~  d  dGNx; i@@$@$@Fr@$@$@ <22@2(  d  d ~{ddG x; i@@$@$@F@$@$@ <22@2(dd ~ d dGx; Ni@@$@$@F@$@$@ <22@2(  dd5}~~   @i` MbP?@@$@ $@ ?j@$@  $@ <22@2(  analyt. curve B6  analyt. curve B $@:@?:@?.K U5   AA5  AB5   61g@?`n@  61g@?`n@  61g@?`n@ 561@U@2@Z@o@  standardput K12 into U5 define standard for standard=10 to 26 put indirect("U"&standard) into K12 recalc put K12 into "AA"&standard put M12 into "AB"&standard end for put U5 into K12 repaint off select object 9 Object Location frac(A128..G152,191,0,1,243) select object 11 Object Location frac(A128..G152,191,0,1,243) select object 10 Object Location frac(A128..G152,191,0,1,243) recalc select object 53 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect  System SystemAanalyt. curve A2b analyt. curve A $@:@?:@?.K U5   AA5  AB5   61g@?`n@  61g@?`n@  61g@?`n@ 561@U@2@Z@o@  standardput I12 into U5 define standard for standard=10 to 26 put indirect("U"&standard) into I12 recalc put I12 into "AA"&standard put M12 into "AB"&standard end for put U5 into I12 repaint off select object 9 Object Location frac(A128..G152,191,0,1,243) select object 11 Object Location frac(A128..G152,191,0,1,243) select object 10 Object Location frac(A128..G152,191,0,1,243) recalc select object 53 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect  System SystemA Insert blank0`^  Insert blank.   #Blank solution inserted               put 1 into Z1 put 0 into O17 put 0 into P17 put "Blank solution inserted" into I16 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 System SystemI numberwheel2/wp !% numberwheel2!U@.5                put ctvalue("numberwheel2",0) into U6 put indirect("U"&6+U6) into K12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 System System??4@? numberwheel.wp   $ numberwheel!U@.5                put ctvalue("numberwheel",0) into U6 put indirect("U"&6+U6) into I12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 System System,@?4@?Remove cuvette'`^ Remove cuvetteG     ."Cuvette removed: Insert to measure               put 0 into X1 put 0 into Z1 put 0 into Q14 put 0 into Q15 put "Cuvette removed: Insert to measure" into I16 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M124 Chicago SystemR Insert samplee&P^  Insert sample8888  h㈵> h㈵> =1000*$I$12 =1000*$K$12$Sample solution inserted               select range I12 put 1 into Z1 put 0.00001 into Q14 put 0.00001 into Q15 put "=1000*$I$12" into O17 put "=1000*$K$12" into P17 put "Sample solution inserted" into I16 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M124 Chicago SystemI$:   Chicago Geneva@,Offset Geneva(( VertSlider#P:$  $%%%%# VertSlider put ctvalue("VertSlider",0) into M8 Chicago Geneva98"@Y@?Y@ ":Y  Chicago Geneva@,Emission wavelength Geneva((!:y Chicago Geneva@,Excitation wavelength Geneva((EmissionSlider :{ p^^^^'EmissionSlider      put ctvalue("EmissionSlider",0) into k8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 Chicago Geneva7V y@i@@?y@ExcitationSlide:pr````)ExcitationSlider     put ctvalue("ExcitationSlider",0) into I8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M12 Chicago Geneva53z)r@i@@?y@ Scan both monoc`   Scan both+$  i@@@@@.'   61g@?`n@  61g@?`n@ 561g@?`n@  61@U@2@Z@o@       countselect range I12 manual recalc define count put I8 into V1 put k8 into V2 for count = 200 to 600 step 2 put count into I8 put count into k8 end for repaint off select object 9 Object Location frac(A128..G152,191,0,1,243) select object 10 Object Location frac(A128..G152,191,0,1,243) select object 53 Object Location frac(A128..G152,191,0,1,243) recalc select object 11 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect put V1 into I8 put V2 into k8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M124 Chicago SystemS Scan EmissionP   Scan Emission  i@@?@?.   61g@?`n@  61g@?`n@ 561g@?`n@  61@U@2@Z@o@       countselect range I12 manual recalc define count put k8 into V2 for count = 200 to 600 put count into k8 end for repaint off select object 9 Object Location frac(A128..G152,191,0,1,243) select object 11 Object Location frac(A128..G152,191,0,1,243) select object 53 Object Location frac(A128..G152,191,0,1,243) recalc select object 10 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect put V2 into k8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M124 Chicago SystemSScan Excitation`  Scan Excitation i@@?@?.  61g@?`n@  61g@?`n@ 561g@?`n@  61@U@2@Z@o@      countselect range I12 manual recalc define count put I8 into V1 for count = 200 to 600 put count into I8 end for repaint off select object 11 Object Location frac(A128..G152,191,0,1,243) select object 10 Object Location frac(A128..G152,191,0,1,243) select object 53 Object Location frac(A128..G152,191,0,1,243) recalc select object 9 Object Location frac(A1..G24,85,18,106,255) repaint on Bring to Front unselect put V1 into I8 recalc range O8..S17 recalc range M10 recalc range M13 recalc range M124 Chicago SystemS$30000uttonD 30 ZZZZ         select chart 9 axis 3 manual scaling from 0 to 30 with 3 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 30 with 3 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 30 with 3 major and 5 minor divisions unselect5 Chicago Geneva3,30000uttonD 3000ZZZZ            select chart 9 axis 3 manual scaling from 0 to 3000 with 3 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 3000 with 3 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 3000 with 3 major and 5 minor divisions unselect5 Chicago Geneva3(30000uttonDx 300 ZZZZ   ,   ,   ,select chart 9 axis 3 manual scaling from 0 to 300 with 3 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 300 with 3 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 300 with 3 major and 5 minor divisions unselect5 Chicago Geneva3OP1:  Chicago Geneva@`,Fluorescence Spectrophotometry Simulation of a two-component mixture. To change the concentrations of the components A and B, click on the up and down arrows to the left of the concentration displays. (Concentration range is zero to 100 ppm in a 1,2, 5, 10 sequence). Or you can type in any concentration while the cuvette is removed. To change the excitation and emission wavlengths (in nm), adjust the two sliders at the bottom. Fluorescence intensity (in arbitrary units) and the absorbance of the solution at the excitation wavelength are displayed in the black boxes. Readings are continuous as long as the cuvette is inserted into the instrument. (The random variations in readings are due to photon noise). To scan a spectrum, click on the corresponding scan button. Change the y-axis scale of the plots by clicking on one of the seven sensitivity buttons below. To obtain a synchronous spectrum, set the wavelength offset with the slider on the right and click "Scan both monochromators". Note: the intensity and absorbance displays respond immediately to changes in concentrations and wavelengths; however, spectra must be re-scanned after changes. Pressing "analyt.curve A" runs an analytical curve for component A and displays a log-log plot of intensity vs concentration of A from 0.001 to 100 ppm. Pressing "analyt. curve B" does the same thing for component B. Scanning a spectrum replaces the analytical curve plot. Geneva Geneva Geneva(()*Auto0uttonD AutoHHHH      select chart 9 axis 3 automatic scaling select chart 10 axis 3 automatic scaling select chart 11 axis 3 automatic scaling unselect 5 Chicago GenevaA,1000ButtonD= 1000ZZZZ         select chart 9 axis 3 manual scaling from 0 to 1000 with 5 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 1000 with 5 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 1000 with 5 major and 5 minor divisions unselect5 Chicago Geneva1(100 ButtonDM 100 ZZZZ   d   d   dselect chart 9 axis 3 manual scaling from 0 to 100 with 5 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 100 with 5 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 100 with 5 major and 5 minor divisions unselect5 Chicago Geneva1)10w Button D& 10ZZZZ             select chart 9 axis 3 manual scaling from 0 to 10 with 5 major and 5 minor divisions select chart 10 axis 3 manual scaling from 0 to 10 with 5 major and 5 minor divisions select chart 11 axis 3 manual scaling from 0 to 10 with 5 major and 5 minor divisions unselect 5 Chicago Geneva1@ @)@H |88,,x,, ~ y be(,, d'KB@ Q54